Figure 2
From: RRHP: a tag-based approach for 5-hydroxymethylcytosine mapping at single-site resolution
Library and sequencing characteristics of RRHP. (a) RRHP libraries prepared with different DNA input, with (+) or without (-) βGT, were digested by either MspI (M) or HpaII (H). L is 100 bp Marker (Zymo Research). (b) MspI digested fragments (green) detected by the RRHP assay overlaid with the fragments (yellow) generated from an in silico simulation. (c) The frequency distribution of 5hmC sites with different read coverage profiled by the RRHP assay.