Figure 4

H3K9 acetylation is altered at Cfp1-regulated regions. (A) Heatmap showing H3K4me3 (yellow) or H3K9,K14ac (blue) normalized read count at TSSs for the most active genes in WT ES cells (n = 2,500). Signal is displayed from -3 kb to +3 kb surrounding each annotated TSS for untreated WT or Cfp1^-/- ES cells. (B) Average profile showing H3K4me3 or H3K9,K14ac normalized read count at TSSs for the most active genes in WT ES cells (n = 2,500). Signal is displayed from -3 kb to +3 kb surrounding each annotated TSS for untreated WT or Cfp1^-/- ES cells. (C) Same as (A) for regions aberrantly accumulating H3K4me3 in absence of Cfp1 (n = 14,208). Signal is displayed from -1.5 kb to +1.5 kb surrounding the center of the peak for each region for untreated WT or Cfp1^-/- ES cells. (D) Average profile showing H3K4me3 or H3K9,K14ac normalized read count for regions aberrantly accumulating H3K4me3 in absence of Cfp1 (n = 14,208). Signal is displayed from -1.5 kb to +1.5 kb surrounding the centre of the peak. Input DNA is plotted as a reference. (E) Comparison of H3K4me3 or H3K9,K14ac normalized read density at TSSs of the most active genes (n = 2,500), from 3 kb upstream to 3 kb downstream. Box plots show the central 50% of the data (filled box), the median (central bisecting line) and 1.5× the interquartile range (whiskers). P-values were calculated using two-tailed unpaired Wilcoxon tests: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (F) Same as (E) for H3K4me3 or H3K9,K14ac normalized read density at regions aberrantly accumulating H3K4me3 in absence of Cfp1 (n = 14,208), from 1.5 kb upstream to 1.5 kb downstream.