Table 2 Primers used in this study for PCR
Plasmid/Gene names | Primers | Sequences |
|---|---|---|
pGEX-Nop58-447 | NOP58-BHI-F | TTGGATCCATGGCTTACGTTTTAACTGAAACTTC |
| Â | NOP58-447-XhoI-R | TTTCTCGAGTTATTCATCATCAGAATCGGATTCAGAATC |
pGEX-Nop58-MC | NOP58-472-BamHI-F | TTGGATCCATGAAAGTTGATGTTATGATTATTCAAGCA |
| Â | NOP58-447-XhoI-R | TTTCTCGAGTTATTCATCATCAGAATCGGATTCAGAATC |
pGEX-Nop58-C | NOP58-853-BamHI-F | TTGGATCCATGCCAAACTTGACTCAGTTGGTTG |
| Â | NOP58-447-XhoI-R | TTTCTCGAGTTATTCATCATCAGAATCGGATTCAGAATC |
pGEX-Nop58-NM | NOP58-BHI-F | TTGGATCCATGGCTTACGTTTTAACTGAAACTTC |
| Â | NOP58-852-XhoI-R | TTTCTCGAGTTAAGCAATAGCCTTCATTCTTGCAG |
p416GAL-GFP-FLAG | GFPs65t-BamHI-F | TTGGATCCATGAGTAAAGGAGAAGAACTTTTCACTGGAG |
| Â | GFP-nonstop-XhoI-R | TTTCTCGAGTTTGTATAGTTCATCCATGCCATG |
p416GAL-Nop58(1–511)-FLAG | NOP58-BHI-F | TTGGATCCATGGCTTACGTTTTAACTGAAACTTC |
| Â | FLAG-XhoI-R | TTTCTCGAGCTATTTATCGTCATCATCTTTGTAGTCCTTGTC |
p416GAL-Nop58(285–511)-FLAG | NOP58-853-BamHI-F2 | TTGGATCCATGCCAAACTTGACTCAGTTGGTTGGTGAAT |
| Â | FLAG-XhoI-R | TTTCTCGAGCTATTTATCGTCATCATCTTTGTAGTCCTTGTC |
p416GAL-Nop58(345–511)-FLAG | NOP58-1033-BamHI-F | TTGGATCCATGGCCTCTCTTGTTGGTCAAGCTACTGGT |
| Â | FLAG-XhoI-R | TTTCTCGAGCTATTTATCGTCATCATCTTTGTAGTCCTTGTC |
p416GAL-Nop58(1–447)-FLAG | NOP58-BHI-F | TTGGATCCATGGCTTACGTTTTAACTGAAACTTC |
| Â | NOP58-447-nostop-XhoI-R | TTTCTCGAGTTCATCATCAGAATCGGATTCAGAATC |
p416GAL-Nop58(285–447)-FLAG | NOP58-853-BamHI-F | TTGGATCCATGCCAAACTTGACTCAGTTGGTTG |
| Â | NOP58-447-nostop-XhoI-R | TTTCTCGAGTTCATCATCAGAATCGGATTCAGAATC |
p416GAL-Nop58(345–447)-FLAG | NOP58-1033-BamHI-F | TTGGATCCATGGCCTCTCTTGTTGGTCAAGCTACTGGT |
| Â | NOP58-447-nostop-XhoI-R | TTTCTCGAGTTCATCATCAGAATCGGATTCAGAATC |
FLAG | FLAG-XhoI-F | TTTCTCGAGATGGATTACAAGGATGACGACGAT |
| Â | FLAG-XhoI-noPae-R | TTTCTCGAGAGCTATTTATCGTCATCATCTTTGTAGTCCTTGTC |
p416GAL-PIH1 | PIH1-BHI-F | TTGGATCCATGGCCGATTTCTTATTGAGACC |
| Â | PIH1-XhoI-R | TTTCTCGAGTTATATATATATATATAGTGTGCGT |
pRS315-PIH1-GFP-NLS | PIH1-m413-XbaI-F | AAATCTAGAGGAAGGTACCAGTGGCAATTCAGCA |
| Â | GFP-NLS-XhoI-R | TTTCTCGAGTTAAACTTTACGTTTCTTCTTTGGAGACTCTTCTTCGGAACCATCAGCAGTACGCTTTTTGTATAGTTCATCCATGCCATGTGTAATCCCAG |
pRS315-PIH1-GFP-mNLS | PIH1-m413-XbaI-F | AAATCTAGAGGAAGGTACCAGTGGCAATTCAGCA |
| Â | GFP-mNLS-XhoI-R | TTTCTCGAGTTAAACTTTACGTTTAGCCTTTGGAGACTCTTCTTCGGAACCATCAGCAGTGGCTGCTTTGTATAGTTCATCCATGCCATGTGTAATCCCAG |
pRS315-PIH1-GFP-NES | PIH1-m413-XbaI-F | AAATCTAGAGGAAGGTACCAGTGGCAATTCAGCA |
| Â | GFP-NES-XhoI-R | TTTCTCGAGTTAAGTCTTATTGATGTCTAAACCAGCTAACTTCAAAGCTAAACCTGGAGCTTTGTATAGTTCATCCATGCCATGTGTAATCCCAG |
pRS315-PIH1-GFP-mNES | PIH1-m413-XbaI-F | AAATCTAGAGGAAGGTACCAGTGGCAATTCAGCA |
| Â | GFP-mNES-XhoI-R | TTTCTCGAGTTAAGTCTTATTAGTGTCAGCACCAGCTAACTTCAAAGCTAAACCTGGAGCTTTGTATAGTTCATCCATGCCATGTGTAATCCCAG |