Figure 7
mpk3 adult plants show reduced flg22-induced callose accumulation and enhanced resistance to virulent P. syringae. (A) Pictures of aniline-blue stained leaves of the indicated phenotypes. Leaves were either untreated (mpk4), infiltrated with mock (H20) or a 1 μM flg22 solution for 24 h. Bar is 200 μm. (B) Relative quantification of callose deposition in leaves of Col-0, mpk3 and mpk6 after infiltration of 100 nM flg22. (C) Pst DC3000 bacterial titres 3 days post spray-inoculation. Bars are means ± SD (n = 5). (D) Pst DC3000 bacterial titres 3 days post inoculation by syringe-infiltration. Bars are means ± SD (n = 4). Letters indicate significant difference based on a Kruskal & Wallis test (α <0.05).