Receiver operating characteristic (ROC) curves comparing deletion prediction performance in the NA12878 individual. The relationship between true positive and false positive calls for deletions in the NA12878 genome is given for LUMPY, GASVPro, DELLY, and Pindel. Each point on a given tool’s ROC curve represents a minimum evidence support threshold ranging from 4 to 11 for 5X coverage and 4 to 20 for 50X coverage. Correctness was determined by two different methods: intersection with one of the 3,376 non-overlapping validated deletions from Mills et al. , or validation by PacBio/Moleculo data. (A,B) As in Figure 5, prediction performance was measured with both 5X mean genome coverage (A) and 50X coverage (B). The curves are colored following the same convention described in Figure 5. LUMPY outperforms all other tools in all but one case. Pindel slightly outperforms LUMPY at higher-evidence thresholds in the 5X coverage case considering the Mills et al. truth set; we note that this is expected given Pindel was used by the 1000 Genomes Project as one of the tools to define this truth set. At the lower coverage, LUMPY’s performance is boosted by the inclusion of either prior evidence or NA12878’s parental genomes, but the read-depth signal is too weak to offer any improvement. The distinction between tools at 50X coverage is low, but it is expected given the coverage and quality of the data. At higher coverage, LUMPY is able to provide a high-confidence call set when considering read-depth, but priors and parental genomes have little added benefit. pe, paired-end; rd, read-depth; sr, split-read.