Figure 3

Sex differences. (A) Sex-specific methylation sites across the genome. The number of cytosines differentially methylated in each 100-kb bin is shown on the Y-axis and the genomic position of the bin is on the X-axis. All sites plotted are significant at 5% FDR, and the horizontal dashed line represents the significance threshold for each bin. (B) Methylation levels across all samples for the gene Lhx9. The height of the bar represents percentage methylation from 0 to 100%. Gray represents missing data. (C) Average methylation levels in C4a at chr17:34960471. (D) Expression levels of C4a in RNA-seq dataset. (E) Expression levels of C4a measured by quantitative PCR. (F) Methylation levels of Selenbp2 at chr3:94499361. (G,H) Expression levels of Selenbp2 in BXD dataset (G) and by quantitative PCR (H). Error bars represent the standard deviation. F, females; M, males.