Figure 3
ChIP-seq analysis (H3K4me1, H3K4me3, HIF1α, PPARβ/δ, H3K27ac) of the ANGPTL4 gene and construct design for the reporter assay. The displayed enrichment scores for ChIP-seq were calculated by QuEST and visualized with IGB software (Affymetrix). (A) ChIP-seq data for H3K4me1, H3K4me3 and HIF1α under normoxia or hypoxia. HUVECs were stimulated with normoxia or hypoxia (1% O2) for 24 hours. The kilobase values show the distance from the TSS of ANGPTL4. (B) Constructs shown in Figure 4 are shown in the frame with their names. Both the constructs were cloned into a pGL3 vector. The displayed hypoxia response element (HRE) motifs (blue arrows and numbers) were estimated by MatInspector (Genomatix). The numbers under the constructs show the distance from the TSS of ANGPTL4 (in base pairs). (C,D) ChIP-seq data for PPARβ/δ (C) and H3K27ac (D) using HUVECs stimulated with the PPARβ/δ agonist (GW501516 100 nM) and/or hypoxia (1% O2) for 24 hours. (E) Basic information for the analysis. The numbers indicate the location on chromosome 19 using the hg18 build. The arrows show the location of primers for ChIP-PCR in Figure 4.