Figure 3

Quantitative reconstruction of leukocyte subsets using a custom, low density DNA methylation microarray. In all panels, the x-axis indicates the quantities of specific WBC subsets determined using DNA methylation. Cell type is indicated by color and sample type is indicated by shape of the point, as described in the inset legends. Lines are drawn from the origin with a slope of one indicating ideal correspondence between the displayed values in each panel. (A) DNA from purified WBC subsets was combined in quantities mimicking human blood under clinical conditions. The expected quantity of each cell type is indicated by the y-axis. (B-D) Whole blood samples from disease-free human donors subjected to WBC subset quantification by established methods. Results of the established methods are shown on the y-axis and include manual 5-part differential (B), automated 5-part differential (C) and FACS (D).