Figure 6

Inverted Alus and site-selective editing in GLI1. (a) UCSC genome browser view. Inverted Alu elements are annotated with their family and strandedness in the repetitive elements by RepeatMasker track, and marked with black arrows. While the most downstream alu (aluSx+) is present in all primates, the two upstream alus (aluJr- and aluY-) are absent in some of them (non-apes), as indicated by the Multiz Alignment track. The non-Alu editing site is shown in a red rectangle. Bottom: The sequence flanking the non-Alu editing site. (b) Sanger sequencing after RT-PCR on GLI1 transcripts from human (hGLI1), rhesus (RmGLI1) and mouse (mGLI1) brains. Editing was detected as a dual A and G peak in the chromatograms. kb, kilobase; R/G, arginine to glycine; RT-PCR, reverse transcription polymerase chain reaction.