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Table 2 Kinetic parameters of the resistant (119 F) and susceptible (L119) GSTe2 alleles

From: A single mutation in the GSTe2 gene allows tracking of metabolically based insecticide resistance in a major malaria vector

 

Vmax(DDE μmol.min−1.mg−1)

K m (μM DDT)

Kcat (s−1)

Kcat/K m (μM−1.s−1)

119 F-GSTe2

60.8 ± 7.2

149.6 ± 39.6

47312 ± 5297

316.3

L119-GSTe2

3.75 ± 0.26

34 ± 5.9

3129 ± 222.6

92.0

  1. Three independent assays were performed for both alleles and the results show mean ± standard deviation.