GSTe2 purification and X-ray three-dimensional structure. (A) Size-exclusion chromatogram of GSTe2 alleles from Benin (BN), Uganda (UG) and Malawi (MAL) alleles. The lines show the absorbance recorded at 280 nm. Molecular-weight markers (Bio-Rad, Hercules, CA, US) are indicated in kilodaltons. (B) SDS-PAGE gel of the three purified GSTe2 alleles (26.8 kDa). (C) Topology of GSTe2 showing the C- and N-terminals, the GSH binding pocket (G-site) and the substrate-binding pocket (H-site). AU, arbitrary units; BN, Benin; GSH, glutathione; MAL, Malawi; MW, molecular weight; UG, Uganda; vol, volume.