Figure 2

Conservation of nuclear organization during development. (A, B) Global association with chromatin is highly similar among NE proteins, both in embryos (A) for LMN-1 (black) and LEM-2 (orange) and during adulthood (B) for LMN-1 (blue) and EMR-1 (green). Embryonic LMN-1 DamID and LEM-2 ChIP MA2C scores were obtained from [34] and [18], respectively. (C, D) Representation of LMN-1 association as chromosomal profiles (C; embryos in black, adults in blue) and boxplot (D; as in Figure 1). (D) LMN-1 MA2C scores in embryos (E-) and adults (A-) averaged over chromosome arms (-A) and centers (-C), or, for the X chromosome, over the left (-L) and right (-R) portions. Probability values from Wilcoxon rank sum tests are indicated: *P < 0.05, ***P < 10^-11. DamID signals at chromosome ends do not differ dramatically between the two developmental stages, whereas chromosome centers are devoid of LMN-1 interaction, particularly in adults. Probe scores were averaged in 100-kb windows for all panels.