Identification of the targets of Smaug-mediated translational repression. (A) The averages, across three biological replicates, of the TI in smaug-mutant and wild-type embryos were plotted against one another. SAM analysis allowed for the identification of 359 transcripts (blue dots) representing 342 genes that show an increase in TI in smaug mutant versus wild type at an FDR of <5%, while the red dots represent genes with FDRs of >5%. The solid diagonal line represents no enrichment and the dotted diagonal lines represent two-fold enrichment or depletion. (B) Polysome gradients from smaug-mutant embryos were performed with or without puromycin treatment and the average, across two biological replicates, of the TI for each gene was calculated. Box plots show the range of TIs for genes where the TI increased in smaug-mutant embryos versus wild type with an FDR <5% and those with an FDR >5%, as defined in (A).