Glycolytic enzymes are overexpressed in smaug -mutant embryos. Extracts were prepared from 0- to 1-, 1- to 2- and 2- to 3-hour old wild-type and smaug-mutant embryos and assayed for (A) hexokinase activity or (B) phosphofructokinase activity. Activities are shown relative to the wild type 0 to 1 hour time point in each case. Results are the average of three independent experiments and error bars indicate standard error of the mean. Data were analyzed using a Student’s t-test (**P < 0.05, *0.05 < P <0.1).