Figure 4

Examples of endonucleolytic cleavages.(a)A group of genes sharing a motif at the cleavage site which was indicated by'↓'. Those identified endonucleolytic cleavages were also found in corresponding biological replicate PARE libraries. (b) PARE reads were shown across the length of mRNAs for endonucleolytic-cleaved genes. Gray bars denoted the coding region of transcripts. The enlarged region showed the sequencing frequencies obtained using RNA Ligase-Mediated (RLM) 5'-RACE assay. The nucleotides in bold letters in the transcripts indicated the cleavages site detected in the PARE analysis. The black arrows indicated digestion sites verified by RLM 5'-RACE with the sequencing frequency (sequencing reads/total sequenced clones) of cloned PCR products. PCR products for the second round of RLM 5' RACE were also indicated, with DL2000 as molecular marker.