Immunostaining of human adult liver for 5mC and 5hmC. Representative stainings of 40 µM adult liver sections are shown. (a) Negative control without the primary antibody. (b) Staining with the monoclonal antibody to 5mC (green) and DAPI (blue). (c) Staining with a polyclonal antibody against 5hmC (red) and DAPI (blue). (d) Merge of 5mC and 5hmC staining and DAPI. As evident from (d), 5mC and 5hmC show similar patterns of intranuclear distribution, indicating that 5hmC, when present, tends to co-localize with 5mC in the hepatocyte nucleus.