Figure 5

Results of vlincRNA RNAi inhibition study. Percent of apoptotic GFP-positive cells (Y-axis) is shown for each indicated treatment (X-axis) for original set of 20 vlincRNAs with 1 siRNA per vlincRNA (A) and for the validation set where 2 additional independent siRNAs per vlincRNA were designed (C). Depletion of selected vlincRNAs was confirmed using real-time RT-PCR (B). "EGFP only" is transfection with EGFP-expressing plasmid only, all other designations imply co-transfection of EGFP-expressing plasmid with the indicated siRNA. Treatments highlighted in yellow are significant at p-value of < 0.05 (Student one-sided t-test). Error bars show standard deviations of 3 independent transfections (A, C) or RT-PCR reaction (B). Results from the negative control siRNA, positive control siRNA against BCR-ABL and AllStars Hs Cell Death positive control siRNA blend are also shown. The vlincRNA data is sorted by the K562-specificity index (see text) with the most K562-specific vlincRNAs on the left (A). Additional details in text and Materials and Methods. The original data is shown in Additional File 3, Table S3.