A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases

Table 5 Mutation frequency of double nuclease pairs versus homology directed repair

	Clone size	Percentage of positive founders	Founders screened (n)	Source
cyc ΔTSS	4.5-22.5%	27.8%	36	This study
sqt ΔWL	3.3-9.5%	10.7%	56	This study
sqt ΔTSS	3.3-6.7%	7.1%	28	This study
apoea -477bp	2-11%	31.3%	16	Gupta et al. 2013 [13]
apoea -4.2kb	1-13%	29.4%	17	Gupta et al. 2013 [13]
th HDR	6.0-29.7%	1.5%	275	Zu et al. 2013 [29]
ponzr1 HDR	NA	~1.6%	186	Bedell et al. 2012 [30]
crhr2 HDR	NA	~13.8%	58	Bedell et al. 2012 [30]

Frequency of deletions in whole locus (ΔWL) and transcriptional start site (ΔTSS) for cyc and sqt compared to large deletions in apoea, and homology directed repair (HDR) at the th, ponzr and crhr2 loci are shown. NA, data not available.

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