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Table 5 Mutation frequency of double nuclease pairs versus homology directed repair

From: A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases

  Clone size Percentage of positive founders Founders screened (n) Source
cyc ΔTSS 4.5-22.5% 27.8% 36 This study
sqt ΔWL 3.3-9.5% 10.7% 56 This study
sqt ΔTSS 3.3-6.7% 7.1% 28 This study
apoea -477bp 2-11% 31.3% 16 Gupta et al. 2013 [13]
apoea -4.2kb 1-13% 29.4% 17 Gupta et al. 2013 [13]
th HDR 6.0-29.7% 1.5% 275 Zu et al. 2013 [29]
ponzr1 HDR NA ~1.6% 186 Bedell et al. 2012 [30]
crhr2 HDR NA ~13.8% 58 Bedell et al. 2012 [30]
  1. Frequency of deletions in whole locus (ΔWL) and transcriptional start site (ΔTSS) for cyc and sqt compared to large deletions in apoea, and homology directed repair (HDR) at the th, ponzr and crhr2 loci are shown. NA, data not available.