Table 1 Mutation frequencies induced by single TALEN pairs
| Â | Â | Mutation frequency by T7E1 in individual embryos | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
TALEN pair | Dosage | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | Mean ± SEM |
egfp5TAL | 12.5 pg | 15.07% | 8.67% | 25.40% | 16.99% | 16.40% | 20.68% | 16.57% | 18.42% | 13.85% | 9.88% | 16.2 ± 1.5% |
egfp3TAL | 12.5 pg | 25.57% | 14.43% | 46.31% | 20.71% | 30.76% | 24.42% | 21.81% | 24.62% | 11.36% | 34.57% | 25.5 ± 3.2% |
sqt5TAL | 25 pg | 8.09% | 7.17% | 7.32% | 4.18% | 11.85% | 8.47% | 6.81% | 12.83% | 10.42% | 13.84% | 9.1 ± 1.0% |
sqt3TAL | 25 pg | 9.77% | 5.91% | 4.89% | 1.90% | 3.46% | 2.00% | 4.37% | 4.44% | 5.48% | 3.22% | 4.5 ± 0.7% |
cyc5TAL | 12.5 pg | 17.53% | 46.86% | 25.65% | 24.72% | 40.45% | 27.34% | 44.29% | 32.57% | 25.27% | 34.58% | 31.9 ± 3.0% |
cyc3TAL | 12.5 pg | 43.76% | 58.13% | 59.00% | 52.41% | 57.46% | 70.59% | 65.23% | 40.71% | 41.66% | 50.57% | 54.0 ± 3.2% |