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Table 1 Mutation frequencies induced by single TALEN pairs

From: A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases

   Mutation frequency by T7E1 in individual embryos
TALEN pair Dosage 1 2 3 4 5 6 7 8 9 10 Mean ± SEM
egfp5TAL 12.5 pg 15.07% 8.67% 25.40% 16.99% 16.40% 20.68% 16.57% 18.42% 13.85% 9.88% 16.2 ± 1.5%
egfp3TAL 12.5 pg 25.57% 14.43% 46.31% 20.71% 30.76% 24.42% 21.81% 24.62% 11.36% 34.57% 25.5 ± 3.2%
sqt5TAL 25 pg 8.09% 7.17% 7.32% 4.18% 11.85% 8.47% 6.81% 12.83% 10.42% 13.84% 9.1 ± 1.0%
sqt3TAL 25 pg 9.77% 5.91% 4.89% 1.90% 3.46% 2.00% 4.37% 4.44% 5.48% 3.22% 4.5 ± 0.7%
cyc5TAL 12.5 pg 17.53% 46.86% 25.65% 24.72% 40.45% 27.34% 44.29% 32.57% 25.27% 34.58% 31.9 ± 3.0%
cyc3TAL 12.5 pg 43.76% 58.13% 59.00% 52.41% 57.46% 70.59% 65.23% 40.71% 41.66% 50.57% 54.0 ± 3.2%
  1. For each single TALEN pair, ten injected embryos were assessed for mutation frequency by the T7E1 assay. SEM, standard error of the mean.