Element | CpGa | Mean MI heavy co-twins | Mean MI light co-twins | Mean MI difference |
P
b
|
|---|
HERVK
| 1 | 0.61 ± 0.05 | 0.63 ± 0.02 | -0.02 ± 0.06 | 0.38 |
| | 2 | 0.36 ± 0.01 | 0.36 ± 0.01 | 0.0008 ± 0.006 | 0.86 |
LINE1 | 1 | 0.58 ± 0.02 | 0.58 ± 0.02 | -0.0007 ± 0.01 | 0.86 |
| | 2 | 0.37 ± 0.02 | 0.38 ± 0.02 | -0.004 ± 0.02 | 0.50 |
- All procedures (including bisulfite treatment, PCR and single-nucleotide primer extension assays in combination with ion-pair reversed-phase high-performance liquid chromatography separation technique (SIRPH) assays) were performed in duplicate; the mean values were used for the statistical analysis. Data are expressed as mean MI ± standard deviation. MI, methylation index; MI difference, MI heavy co-twin - MI light co-twin. aCpG 1 and 2 corresponds to the CpG tagged by SNuPE primer 1 and 2, respectively. bHeavy versus light calculated using a Wilcoxon signed-rank test.
