Figure 9
Small molecule inhibition of FSH induces widespread down-regulation of gene expression. S2-DRSC cells were treated using the BET inhibitor JQ1 for 1, 2, 4, and 20 h. For each time-point relative gene expression changes were monitored by mRNA-seq. (A) MA plot showing gene expression changes after 4 h of JQ1 treatment. Red color highlights genes showing a significant differential expression (adjusted P value <0.05). (B) Scatterplot of gene expression changes versus FSH enrichments at promoters. (C) Heat map illustrating expression changes over time for all genes >2 times up- or down-regulated after 4 h of treatment. Colors indicate regularized log2 fold change according to color key. (D) Bar plots summarizing the number of up- and down-regulated genes for each time point. (E) JQ1 pretreatment of S2-DRSC cells dampens LPS-inducible gene expression. CecA1 and CecB transcript levels were measured using qPCR. (F) Removal of FSH from active promoters by JQ1 treatment is accompanied by a loss of ASH1. Bar charts show ChIP signals measured by qPCR. Error bars indicate STDs of two biological replicates. The TSS of CG6280 is neither occupied by ASH1, nor by FSH according to ChIP-seq analysis (represents background signal).