Figure 4

NETs promote movement of whole chromosomes independently of the lacO array. (a) NET-transfected cells subjected to FISH with whole chromosome paints and a probe for lacO. For NET39 both copies of chromosome 5 were in direct proximity to the NE, whereas the singly integrated array was merely closer; thus, relocalization to the periphery is independent of lacO sequences. (b) FISH with chromosome 5 paint on NET transfected HT1080 cells lacking a lacO array validated the lacO screen results. (c) Average DAPI fluorescence intensity in each nuclear erosion shell. No differences in DAPI distribution could be observed between the control cells and the NET transfected cells. n = 50 cells for each NET. (d) Quantification of chromosome 19 pixel intensity in the shells generated by the erosion macro. For the graph the percentage of the total intensity in the two outermost shells is summed (1+2, peripheral) and plotted against the summed percentage of the total intensity in the two innermost shells (4+5, internal). Error bars indicate standard deviation between the means of individual experiments. For both (a) and (b) scale bar = 5 μm.