Figure 1

CTCF ChIP-seq in seven primate species. (A) Lymphoblastoid cell lines (LCLs) from seven primate species: Homo sapiens (Hsap), Pan troglodytes (Ptro), Gorilla gorilla (Ggor), Pongo pygmaeus (Ppyg), Macaca mulatta (Mmul), Papio hamadryas (Pham) and Sanguinus Oedipus (Soed) were formaldehyde cross-linked prior to CTCF ChIP-seq experiments. YY1 ChIP-seq was performed in a subset of primates - Hsap, Ptro, Ppyg and Soed. Sequencing reads were aligned to each respective species' genome and peaks called at a fixed false discovery rate (FDR). Inter-species comparisons were based on the EPO multiple sequence alignments. (B) A primate-shared CTCF binding event at the MRPL39 gene, and a human-specific binding event within the COQ7 gene in LCLs from each primate species [representing the great ape, Old World monkey (OWM) and New World monkey (NWM) clades] as well as Mus musculus (Mmus) liver.