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Table 1 Details of the treatments used for RNA sequencing analysis of the tomato immune response

From: Transcriptomics-based screen for genes induced by flagellin and repressed by pathogen effectors identifies a cell wall-associated kinase involved in plant immunity

Treatment Concentration Comment
flgII-28 1 μM 28-amino acid peptide from Pseudomonas syringae pv. tomato T1
Pseudomonas syringae pv. tomato (Pst) DC3000 5 × 106 cfu/mL Wild-type Pst strain
DC3000ΔhrcQ-UΔfliC 5 × 106 cfu/mL DC3000 mutant, lacking a functional T3SS and flagellin
DC3000ΔavrPtoΔavrPtoB 5 × 106 cfu/mL DC3000 mutant, with a functional T3SS, but lacking avrPto and avrPtoB
Pseudomonas fluorescens 55 108 cfu/mL Soil bacterium
Pseudomonas putida KT2240 108 cfu/mL Soil bacterium
Agrobacterium tumefaciens GV2260 108 cfu/mL Disarmed plant pathogen
MgCl2 10 mM Mock treatment
- - Untreated plants
  1. T3SS: type III secretion system; cfu: colony-forming units.