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Table 1 Details of the treatments used for RNA sequencing analysis of the tomato immune response

From: Transcriptomics-based screen for genes induced by flagellin and repressed by pathogen effectors identifies a cell wall-associated kinase involved in plant immunity

Treatment

Concentration

Comment

flgII-28

1 μM

28-amino acid peptide from Pseudomonas syringae pv. tomato T1

Pseudomonas syringae pv. tomato (Pst) DC3000

5 × 106 cfu/mL

Wild-type Pst strain

DC3000ΔhrcQ-UΔfliC

5 × 106 cfu/mL

DC3000 mutant, lacking a functional T3SS and flagellin

DC3000ΔavrPtoΔavrPtoB

5 × 106 cfu/mL

DC3000 mutant, with a functional T3SS, but lacking avrPto and avrPtoB

Pseudomonas fluorescens 55

108 cfu/mL

Soil bacterium

Pseudomonas putida KT2240

108 cfu/mL

Soil bacterium

Agrobacterium tumefaciens GV2260

108 cfu/mL

Disarmed plant pathogen

MgCl2

10 mM

Mock treatment

-

-

Untreated plants

  1. T3SS: type III secretion system; cfu: colony-forming units.