Experimental validation of liver enhancer predictions using the hydrodynamic tail vein enhancer assay. On each injection day, we also injected an empty pGL4.23[luc2] vector and a known liver enhancer of the ApoE gene as negative and positive controls, respectively. At least three mice were injected per construct. Statistical significance was tested using Student’s t-test followed by multiple testing adjustment with Benjamini-Hochberg’s method. The asterisks indicate statistical significance to control at adjusted P-value ≤0.05.