Genome-wide analysis of pH
in growing yeast mutant cultures. (a) Distribution of average pHc values of 1,068 wild-type and 4,208 deletion mutant strains in two biological replicate screenings of exponentially growing cultures. (b) pHc mutants identified in primary and secondary screens. Significantly deviating mutants (P-value < 0.01) were identified after six biological replicate determinations of pHc in cultures 4 hours after initiation of growth on glucose. (c) Hypergeometric enrichment of Gene Ontologies in pHc mutants. Fold enrichment is defined as the number of mutants in a given ontology in the pHc mutant dataset relative to the number of mutants in that ontology in the screened background dataset (4,740 mutants). *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001.