Transcription of pseudogenes. (a) Pipeline for computational identification of transcribed pseudogenes (Pgenes). The 'OR' gate (binary operator) indicates the acceptance criteria for a candidate to enter the transcribed pseudogene pool. Expressed pseudogene candidates showing transcription evidence in ESTs/mRNAs, total RNA-Seq data, and BodyMap data were sent for wet-lab validation by RT-PCR or RT-PCR-Seq. (b) Process flow of experimental evaluation of pseudogene transcription. (c) User interface of PseudoSeq for identifying transcribed pseudogenes with BodyMap data. (d) Transcribed pseudogenes identified using Human BodyMap data. (e) Experimental validation results showing the transcription of pseudogenes in different tissues.