Figure 4

Enhanced cleavage at intronic poly(A) sites in proliferation. (a) Two examples of genes that showed significant enhancement of cleavage at intronic poly(A) sites in proliferative MCF10A cells compared to serum-starved ones. Ratios between intronic and 3' UTR peak levels are indicated to the right. (b) Global enhancement of cleavage at intronic sites was associated with proliferation in both BJ cells (left, proliferative compared to confluent cells) and MCF10A cells (right, proliferative compared to serum starved cells). (c) Enhanced cleavage at intronic sites in proliferation was validated using 3'-end qRT-PCR applied to intronic and 3' UTR distal poly(A) sites of RNF220 and FAM70B, measured in MCF10A under proliferative and serum-starved conditions. The figure shows means (± standard deviation) of three independent experiments (for both genes, ^**P < 0.005; one-tailed t-test).