Figure 4

The co-targeting sequence of miR858 and miR828 is located in the region encoding the R3 repeat domain of MYB proteins. (a) Schematic diagram of MYB proteins, and the location of the co-targeting region of miR828 and miR858. The MYBs containing a single or multiple R domains (R1, R2 and R3) are indicated. H, helix; T, turn; W, tryptophan; X, amino acid. (b) Distribution of alignment score (AS) between miRNAs (miR828 and miR858) and their target sites among 251 apple (upper pie charts) and 129 Arabidopsis MYB genes (lower pie charts). AS categories: ≤5, cleavage-favorable; 5 < AS ≤ 7, less cleavage-favorable; > 7, cleavage-unfavorable. (c) Conservation of the H3 helix and its flanking sequences among 251 apple MYB proteins. The conservation of amino acid residues in each position is denoted with various colors: dark color indicates a high level; light color indicates a low level of conservation. (d) Conservation of 18 amino acid residues encoded by the miR828 and miR858 co-targeting region. The bit value representing the conservation in each position is indicated. (e) Conservation of the 55-nucleotide co-targeting region in 251 apple MYB genes. The pairing between the consensus sequence and each miRNA is illustrated. miRNA target sites, the H3 coding region and the miRNA seed region are also marked.