Copy number variation among E. histolytica genes. Coverage depth of a set of 6,228 E. histolytica genes relative to HM-1:IMSS in eight sequenced strains. The y-axis displays the ratio of a strain's RPKM to the average RPKM of HM-1A and HM-1B. The x-axis represents 6,228 annotated E. histolytica genes ordered by their amoebaDB gene ID. Ratios greater than 10 were truncated for display purposes. Grey bars represent results for all uniquely aligned reads, black bars for uniquely aligned reads with unique start positions on the reference genome (see Materials and methods).