Figure 2

Small size mutants are defective in the signaling of different G2/M regulatory pathways. (a) Genetic interactions between identified mutations and the cdr1 and sty1 genes. Horizontal axis represents the ratio of cell length at division of the double mutant of each identified mutation in combination with sty1Δ over the single sty1Δ mutant. The vertical axis represents the cell length ratio of the double mutant with cdr1Δ over the single cdr1Δ mutant cell length. Shaded areas indicate genes that showed interaction with either sty1Δ or cdr1Δ. (b) Distribution of cell length at division and pictures of Blankophor-stained cells of the wild type (wt) and cdr1Δ sty1Δ double mutant strains. Mean cell length values ± standard deviation (n = 97) are indicated. (c) Cell length at division of triple mutants carrying sty1Δ and cdr1Δ deletions, and gene deletions that were additive to either sty1Δ or cdr1Δ. P-value was determined by Student's t-test. (d) Genetic interactions within the group of mutations that reduce cell size of the cdr1Δ sty1Δ double mutant. In the figure, additive phenotype means that the double is significantly smaller (P < 0.01) than both single parental strains.