Figure 4

Classes of transcript profiles during the MZT in PGCs. (a) Decision tree defining the different transcript classes. Expression profiles of transcripts in wild-type PGCs. Class I: transcripts that decrease in level at both the 3-to-5 hour and the 5-to-7 hour time points. Class II: transcripts that decrease in level at 3-to-5 hour but then do not change in abundance at the 5-to-7 hour time point. Class III: transcripts that decrease in level at 3-to-5 hour but then increase in level at the 5-to-7 hour time point. Class IV: transcripts that are present at the same level at the 1-to-3 and 3-to-5 hour time points, but then decrease at the 5-to-7 hour time point. Class V: transcripts that are present at the same level throughout the time course. Class VI: transcripts that are present at the same level at 1-to-3 and 3-to-5 hours, but increase in level at the 5-to-7 hour time point. Class VII: transcripts that increase in level at 3-to-5 hours but then decrease in level at the 5-to-7 hour time point. Class VIII: transcripts that increase in level at 3-to-5 hours, and then remain at the same level at the 5-to-7 hour time point. Class XI: transcripts that increase in level at both the 3-to-5 and 5-to-7 hour time points. (b) Expression profiles in wild-type PGCs of the transcripts in these nine classes across the 1-to-3, 3-to-5 and 5-to-7 hour time points. (c) Expression profiles of transcripts in Classes I to IX in smaug-mutant PGCs. In (b, c) each line represents the average expression profile of a transcript from all the replicates.