Venn diagram comparing our lists of proteins and RNAs to previous studies. (a) Venn diagram showing proteins detected in 1-to-3 hour soma and PGCs that have previously been reported to be present in 0-to-1.5 and 3-to-4.5 hour old fly embryos . (b) Venn diagram showing overlap between our PGC-enriched and soma-enriched transcripts relative to ones previously reported as 'pole cell localized at stages 4 to 6' in the BDGP in situ database  (105 genes in total). (c) As in (b), but comparing our transcripts with those annotated as 'pole cell enriched at stages 4 to 5' in the Fly-FISH database  (230 genes in total). The PGC-enriched and soma-enriched transcripts were determined by comparing the expression profiles of 1-to-3 hour GFP-positive and GFP-negative cells (Additional file 9).