Table 1 Predicted knockout strategies for Astaxanthin over-production in cyanobacteria
Organism | Growth rate (mutant/wt) [h-1] | Astaxanthin production [μmol gDW-1 h-1] (min/max) | Knockout reaction names | KEGG reaction ID/EC number |
|---|---|---|---|---|
P. marinus as9601 | 0.026/0.061 | 2.18/2.18 | Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase | R01658/EC 2.5.1.1 |
| Â | Â | Â | 5-O-(1-Carboxyvinyl)-3-phosphoshikimate phosphate-lyase | R01714/EC 4.2.3.5 |
T. elongatus | 0.016/0.048 | 2.08/2.08 | Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase | R01658/EC 2.5.1.1 |
| Â | Â | Â | Phosphoenolpyruvate: D-erythrose-4-phosphate C-(1-carboxyvinyl)transferase | R01826/EC 2.5.1.54 |
G. violaceus | 0.033/0.061 | 1.64/1.64 | Succinate:(acceptor) oxidoreductase | R00408/EC 1.3.99.1 |
| Â | Â | Â | Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase | R01658/EC 2.5.1.1 |
Synechococcus pcc7942 | 0.033/0.061 | 1.64/1.64 | Succinate:(acceptor) oxidoreductase | R00408/EC 1.3.99.1 |
| Â | Â | Â | Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase | R01658/EC 2.5.1.1 |
Cyanobacteria cyb | 0.032/0.058 | 1.57/1.57 | Succinate:(acceptor) oxidoreductase | R00408/EC 1.3.99.1 |
| Â | Â | Â | Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase | R01658/EC 2.5.1.1 |
T. erythraeum | 0.051/0.061 | 0.24/0.24 | Hydrogen-carbonate: L-glutamineamido-ligase | R00575/EC 6.3.5.5 |
| Â | Â | Â | Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase | R01658/EC 2.5.1.1 |
A. variabilis | 0.051/0.061 | 0/0.96 | 4-Methyl-2-oxopentanoate: NAD+ oxidoreductase | R01651/EC 1.2.1.25 |
| Â | Â | Â | 1-Deoxy-D-xylulose-5-phosphate pyruvate-lyase | R05636/EC 2.2.1.7 |
Anabaena | 0.051/0.061 | 0/0.96 | 3-(4-Methylpent-3-en-1-yl)-pent-2-enedioyl-CoA hydrolyase | R03493/EC 4.2.1.57 |
| Â | Â | Â | 1-Deoxy-D-xylulose-5-phosphate pyruvate-lyase | R05636/EC 2.2.1.7 |