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Table 1 Predicted knockout strategies for Astaxanthin over-production in cyanobacteria

From: MIRAGE: a functional genomics-based approach for metabolic network model reconstruction and its application to cyanobacteria networks

Organism Growth rate (mutant/wt) [h-1] Astaxanthin production [μmol gDW-1 h-1] (min/max) Knockout reaction names KEGG reaction ID/EC number
P. marinus as9601 0.026/0.061 2.18/2.18 Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase R01658/EC 2.5.1.1
    5-O-(1-Carboxyvinyl)-3-phosphoshikimate phosphate-lyase R01714/EC 4.2.3.5
T. elongatus 0.016/0.048 2.08/2.08 Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase R01658/EC 2.5.1.1
    Phosphoenolpyruvate: D-erythrose-4-phosphate C-(1-carboxyvinyl)transferase R01826/EC 2.5.1.54
G. violaceus 0.033/0.061 1.64/1.64 Succinate:(acceptor) oxidoreductase R00408/EC 1.3.99.1
    Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase R01658/EC 2.5.1.1
Synechococcus pcc7942 0.033/0.061 1.64/1.64 Succinate:(acceptor) oxidoreductase R00408/EC 1.3.99.1
    Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase R01658/EC 2.5.1.1
Cyanobacteria cyb 0.032/0.058 1.57/1.57 Succinate:(acceptor) oxidoreductase R00408/EC 1.3.99.1
    Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase R01658/EC 2.5.1.1
T. erythraeum 0.051/0.061 0.24/0.24 Hydrogen-carbonate: L-glutamineamido-ligase R00575/EC 6.3.5.5
    Dimethylallyl-diphosphate: isopentenyl-diphosphate dimethylallyl transtransferase R01658/EC 2.5.1.1
A. variabilis 0.051/0.061 0/0.96 4-Methyl-2-oxopentanoate: NAD+ oxidoreductase R01651/EC 1.2.1.25
    1-Deoxy-D-xylulose-5-phosphate pyruvate-lyase R05636/EC 2.2.1.7
Anabaena 0.051/0.061 0/0.96 3-(4-Methylpent-3-en-1-yl)-pent-2-enedioyl-CoA hydrolyase R03493/EC 4.2.1.57
    1-Deoxy-D-xylulose-5-phosphate pyruvate-lyase R05636/EC 2.2.1.7
  1. For each species the table shows the predicted reactions whose knockout is expected to provide maximal astaxanthin production rate, the expected astaxanthin production rate, and the expected decline in growth rate.