Comprehensive comparison of three commercial human whole-exome capture platforms

Table 2 Uniformity of depth by three human exome capture platforms

	Filtered		Mean coverage (×)		Coverage depth (percent of bases in TR)				Coverage depth (percent of bases in FR)
Replicate^a	Reads (M)	Bases (Mb)	On TR	On FR	0×	1× to 10×	10× to 50 ×	>50×	0×	1× to 10×	10× to 50×	>50×
NA-r1	25.5	2,280	30.8	9.3	1.3	12.3	69.8	16.6	6.8	56.2	36.8	0.2
NA-r2	27.1	2,421	30.0	8.5	1.0	11.2	73.8	14.1	7.4	53.1	33.1	0.1
NS-r1	26.1	2,338	30.1	10.8	1.7	13.0	69.7	15.6	6.1	53.1	39.5	1.3
NS-r2	25.5	2,289	30.3	9.9	1.5	13.6	68.5	16.3	6.7	56.4	35.8	1.0
AS-r1	25.7	2,222	32.7	3.5	2.1	18.6	59.5	19.8	46.8	42.7	10.1	0.4
AS-r2	25.1	2,175	32.5	3.4	2.2	19.0	59.0	19.8	47.3	42.4	9.9	0.4

For the analyses, a set of data that has 30-fold coverage on targeted regions was randomly selected for each of the six replicates. ^aAS, Agilent solution; NA, NimbleGen array; NS, NimbleGen solution; r1 and r2 are two replicate experiments for each platform. FR, flanking region; TR, targeted region.

ISSN: 1474-760X