Figure 4
Respirometric analysis of PPARδ agonist-treated 3T3-L1 adipocytes. (a) Graph showing the respiratory rates of in situ permeabilized control (n = 3) and PPARδ agonist-treated (n = 3) 3T3-L1 cells performing β-oxidation using palmitoyl-carnitine measured using a Clark-type oxygen electrode. *P = 0.05. (b) Graph showing the respiratory rates of the electron transport chain complex IV of in situ permeabilized control (n = 3) and PPARδ agonist-treated (n = 3) 3T3-L1 cells measured using a Clark-type oxygen electrode. *P < 0.05. (c) Spectrophotometric measurement at 510 nm of Oil Red O eluted from stained 3T3-L1 cells treated with DMSO control (n = 3) or 100 nM (n = 3) or 1 μM (n = 3) of the PPARδ agonist GW610742. Error bars represent standard errors of the mean.