Farnesyltransferase inhibitor treatment restores chromosome territory positions and active chromosome dynamics in Hutchinson-Gilford progeria syndrome cells

Table 2 Distribution of NM1β in HGPS cells after a 48 hour FTI treatment

	Proliferating HGPS cells + FTI	0 hours (quiescent HGPS cells + FTI)	24 hours	36 hours	48 hours
Nucleolar + rim + nucleoplasmic	73.7 ± 7.09	2.1 ± 1.53	35.0 ± 8.18	51.6 ± 3.61	64.0 ± 4.0
Nucleolar only	0 ± 0	19.3 ± 11.32	14.9 ± 2.0	9.8 ± 3.46	8.5 ± 2.64
Aggregates	18.6 ± 6.55	74.2 ± 5.29	45.8 ± 1.53	31.5 ± 4.58	21.8 ± 2.51
Rim + nucleoplasmic	4.8 ± 2.08	0 ± 0	2.5 ± 1.53	3.8 ± 2.08	1.6 ± 0.06
Nucleoplasmic	1.8 ± 2.08	2.9 ± 2.08	1.2 ± 1.15	2.6 ± 3.21	2.1 ± 2.64
Dull	0.9 ± 1.53	1.5 ± 1.53	0.4 ± 1.73	0.5 ± 2.31	1.9 ± 3.79

Distribution was analyzed in FTI-treated HGPS cells in 15% serum (proliferating), 0.5% serum for 7 days (quiescent) and 24, 36 and 48 hours after the re-addition of serum. The distribution of NM1β in proliferating cells was more like control fibroblasts with a normal distribution of nucleolar + rim + nucleoplasmic that became like normal quiescent cells with the withdrawal of serum and returned to a more normal distribution for proliferating cells within the expected time frame (36 to 48 hours) [42]. Numbers are percentage values with ± standard deviation.

ISSN: 1474-760X