Single cell transcriptomics of neighboring hyphae of Aspergillus niger

Table 1 Accumulation of RNA is heterogeneous between hyphae at the periphery of an A.niger colony

Gene	Sample type	μCt	σ	σ technical
18S	1 hypha	20.23	2.40	0.53-1.26
	100 hyphae	17.39	0.42	0.26-0.43
	5 pg periphery	12.58	0.28	0.15-0.35
actin	1 hypha	30.30	6.07	0.11-1.03
	100 hyphae	28.15	4.80	0.12-1.71
	5 pg periphery	18.05	0.57	0.09-0.22
glaA	1 hypha	25.47	4.63	0.06-0.66
	100 hyphae	24.51	2.22	0.10-0.22
	5 pg periphery	18.65	0.49	0.10-0.14

QPCR was performed using 1 ng of cDNA amplified from RNA of tips of single neighboring hyphae from the outer periphery of a sandwiched colony, from RNA of a pool of 100 of such tips, and from RNA of the 3-mm-wide periphery of sandwiched colonies of A. niger. The average cycle threshold (μCt) and their standard deviations (σ) are given for 18S rDNA and the actin and glaA genes. σ technical represents the range in standard deviations obtained for the six technical replicates for each of the biological replicates.

ISSN: 1474-760X