Gene fragmentation in NGS assemblies. Gene ENSGALG00000006569 locates from 16,937,180 to 17,042,224 on chromosome 13 in the Gallus-gallus-2.1 reference. The gene is broken into six scaffolds in the Illumina/SOAP assembly, and four scaffolds in the 454/Newbler assembly. Green bars represent scaffolds in the Illumina/SOAP assembly, and red bars represent scaffolds in the 454/Newbler assembly. Solid colored bars within scaffolds represent aligned regions while open bars denote gaps. The percentage GC (%GC) plot shows the relative GC content along the genome sequence. The horizontal red line indicates 50% GC content.