Two forms of conservation on disorder. Schematic of computing disorder conservation and amino acid (AA) sequence conservation. After alignment, the percentage of sequences in which a residue is disordered is computed. Similarly, we compute the percentage of sequences in which the amino acid itself is conserved. A residue is considered to be conserved disorder if the property of disorder is conserved in ≥ 50% of species and sequentially conserved if the amino acid is conserved in ≥ 50% of species. Disordered residues in which both sequence and disorder are conserved are referred to as constrained disorder. Disordered residues in which disorder is conserved but not the amino acid sequence are referred to as flexible disorder. Residues which are disordered in S. Cerevisiae but not cases of conserved disorder are referred to as non-conserved disorder.