Figure 4
HPL-2 modulates lifespan by acting through the daf-2 /IIS pathway. (a) Survival curve of wild-type and hpl-2(tm1489) animals. hpl-2(tm1489) mutation increases average lifespan by 17% (average lifespan: hpl-2(tm1489), 15.1 ± 0.31 days (n = 287); wild type (WT), 12.9 ± 0.25 days (n = 280); log rank test P < 10-3). (b) Survival curve of daf-2(e1370);hpl-2(tm1489) double mutants compared to daf-2(e1370) single mutants. hpl-2(tm1489) did not further increase daf-2(e1370) mutant lifespan (average lifespan daf-2(e1370), 26.2 ± 0.85 days (n = 95); daf-2(e1370);hpl-2(tm1489), 24.8 ± 0.97 days (n = 84); log rank test P = 0.271.). (c) Survival curve of daf-16(mu86);hpl-2(tm1489) compared to daf-16(mu86). daf-16(mu86) mutation suppressed hpl-2(tm1489) mutant lifespan phenotype (average lifespan: daf-16(mu86), 11.9 ± 0.44 days (n = 112); daf-16(mu86);hpl-2(tm1489), 11.03 ± 0.27 days (n = 109); log rank test P = 0.05).