Figure 2

HPL-2 binding to target genes. Worm extracts from synchronized L3 worms were subjected to immunoprecipitation (IP) using either HPL-2-specific antibodies or pre-immune serum as control. For each gene tested, a cartoon shows the position of the primers used for quantitative PCR (qPCR) analysis and a histogram representing the fold-enrichment, calculated as the ratio between signal from the antibodies and signal from pre-immune serum, from chromatin immunoprecipitation (ChIP) experiments along the gene. chIV is a primer from an intergenic region on chromosome IV used as an internal control. Similar results were obtained for two to three independent experiments.