Figure 3

Ribosomal protein co-regulation and functional enrichment analysis of protein expression ratios. (a) Box and whisker plot of the log₂ ratios of stationary over exponential phase for 51 ribosomal proteins from the low solubility protein fraction. Boxes designate the quartiles. The thick centerline designates the median. Thin lines illustrate the range. (b) A graphical representation of the functional enrichment analysis method from [19] applied to protein expression ratios comparing stationary to exponential phase of growth in E. coli computed by our method. In this representation, rows correspond to significantly informative pathways and columns correspond to 10 equally populated expression bins of log₂ ratios (stationary over exponential). Colors indicate pathway over or under-representation levels across the expression bins. Red designates (in log₁₀) over-representation of a particular pathway in any expression bin, whereas, blue designates under-representation.