Relaxed pipeline for smORF search. Pipeline for search of smORFs with the lowered E-value < 0.05 threshold for the tBLASTn filter. Despite an initial higher percentage of smORFs passing this filter, subsequent results are similar to those obtained by the initial stricter pipeline shown in Figure 1. For details see text and Materials and methods. Dp, D. pseudoobscura; Ka/Ks, ratio of non-synonymous (Ka) to synonymous (Ks) nucleotide substitution.