Figure 5

Fruiting body development, sexuality and mating-type analysis. (a-c) Chinese Tussah silkmoth pupae were inoculated with conidia from the C. militaris Cm01 strain and incubated for 14 days (a), 29 days (b) and 59 days (c) to produce nascent, mid-term and developmentally mature fruiting bodies. (d-g) The mature fruiting bodies of the Cm01 strain do not produce perithecia (d, e) but those of strain Cm06 are completely covered with protruded perithecia (f, g). (h) PCR examination of different strains (numbers labeled on the top) showed that strains Cm06, Pm36 and 80399 contain the MAT1-1-1, MAT1-1-2 and MAT1-2-1 genes while Cm01 and other strains lack the MAT1-2-1 gene. (i) PCR examination of 30 randomly selected single spore isolates from the hybrid strain Cm06 showed that only 2 out of 30 isolates contain the MAT1-2-1 gene.