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Table 2 Mutations identified in R6M1 and R6M2 penicillin-resistant mutants

From: Whole genome sequencing of penicillin-resistant Streptococcus pneumoniae reveals mutations in penicillin-binding proteins and in a putative iron permease

   S. pneumoniaestrain
   M1 M2
Locus name Putative identification Nucleotide change Amino acid change Nucleotide change Amino acid change
spr0032 DNA polymerase I    A107G H36R
spr0041 Transposase (orf2)    GAA31AAC E11N
     GCTCG36TCTCA K12N;D14N
     T63A SYN
     GG204TA D69Y
spr0113 Hypothetical protein    A4G K2E
spr0121 Surface protein PspA precursor    A4G N2D
     T1374C SYN
spr0160 DNA mismatch repair protein (Hex B)    G752A S251N
spr0182 Hypothetical protein    G82insertion frame shift
spr0284 Alpha-xylosidase    T1107C SYN
spr0304 Penicillin-binding protein 2x A1150G R384G A842C Q281P
   G1552T V518L C1106T A369V
   C1654G Q552E A1150G R384G
     C1276T R426C
     G1552A V518I
spr0329 Penicillin-binding protein 1a G1630A G544R G1233A W411*
spr0376 Conserved hypothetical protein    T207C SYN
spr0422 Hypothetical protein    C391T Q131*
spr0475 Conserved hypothetical protein G318C L106F   
spr0509 Phenylalanyl-tRNA synthetase beta chain    C105T SYN
spr0598 GTP-binding protein (TypA/BipA) (tyrosine phosphorylated protein A)    C905T P302L
spr0666 ABC transporter ATP-binding protein - cell division (FtsE) C410T P137L   
spr0764 30S Ribosomal protein S1    G1173A M391I
spr0776 D-Alanyl-D-alanine carboxypeptidase    C287T A96V
spr0866 Dihydroorotate dehydrogenase C575T P192L   
spr0878 Exoribonuclease R G280T G94W   
spr0895 Conserved hypothetical protein    G134C R45T
spr0917 Citrulline cluster-linked gene C59T A20V   
spr0934 ABC transporter substrate-binding protein - iron transport    G486A W162*
spr1041 Hypothetical protein C648T SYN   
spr1043 Conserved hypothetical protein C109T P37S C105T SYN
spr1092 tRNA pseudouridine 5S synthase    C210T SYN
spr1127 Ribonuclease III A228G SYN C554T T185I
spr1152 LicD G692T P231L   
spr1166 Signal recognition particle (Fifty four homolog) C139T P47S   
spr1178 Hypothetical protein C82T Q28* C82T Q28*
spr1186 N-Acetylneuraminate lyase subunit, truncation    T603C SYN
     G620A G207D
     GAC660AAT T221I
     AA689CG E230A
     T696A SYN
spr1224 Conserved hypothetical protein    T564C SYN
spr1240 Alanyl-tRNA synthetase    A139G T47A
spr1254 ABC transporter ATP-binding protein-phosphate transport (PstB) G499A G167S C614T T205I
spr1260 Conserved hypothetical protein    T597C SYN
spr1272 N-Acetylglucosamine-6-phosphate isomerase G385A G129R   
spr1384 UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine ligase G996A M332I   
spr1423 Conserved hypothetical protein    A673G M225V
spr1453 Major facilitator superfamily transporter T91C F31L   
spr1465 Conserved hypothetical protein G419A G140E   
spr1517 Penicillin-binding protein 2b C1245A D415E C1184T A395V
   C1528A G665D G1303A G435S
     A1351G T451A
spr1587 Conserved hypothetical protein C568T SYN   
spr1703 ABC transporter ATP-binding protein - oligopeptide transport C575A P192Q   
spr1706 ABC transporter membrane-spanning permease - oligopeptide transport    T399deletion frameshift
spr1862 Competence protein    A51G SYN
spr1886 Degenerate transposase A314G *105W   
spr1888 DNA mismatch repair protein (HexA) C2183T T728I C976T Q326*
spr1991 Glycerol kinase    GG78TT E27*
  1. Asterisks and SYN indicate nonsense mutations and synonymous mutations, respectively.