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Table 3 Performance comparison of manual versus automated solution hybrid selection

From: A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries

Factor Column based Automated (with-bead SPRI) Automated (with-bead SPRI) low input
Input DNA 3 μg 3 μg 0.1 μg
Samples/FTE/week 6-12 384 384
Number of sample transfer steps 10 4 4
Output DNA prior to PCR 720 ng 1,330 ng Below limit of detection
Number of pond PCR cycles 12-16 6 6
Percentage duplicated reads 19.8 2.2 10
Percentage selected bases 84.7 88.6 83.76
Estimated library size 43 million 516 million 223 million
  1. FTE, Full time employee; SHS, solution hybrid selection; SPRI, solid-phase reversible immobilization.