Figure 3
From: Evolution of an adenocarcinoma in response to selection by targeted kinase inhibitors
Fluorescent in situ hybridization (FISH) and immunohistochemical analysis of the sublingual adenocarcinoma. (a) Hematoxylin and eosin stained section of tumor (20× objective). (b) Striking amplification of RBBP8 (40×, with RBBP8 probe in red). (c) Focal nuclear and cytoplasmic expression of PTEN (20×) is associated with (d) a missing red signal indicating monoallelic loss of PTEN (100×; the orange gene-specific probe signals are decreased in number compared to the centromeric probe). (e) Diffuse, strong cytoplasmic expression of RET (20×) is associated with (f) amplification of the RET gene (40× with bacterial artificial chromosomes flanking the RET gene labeled in red and green).