Figure 6

PolII can still be recruited even after shifting rpb1-1 to 37°C. (a-c) Examples of time course data from cells shifted to 37°C (left panel), treated with diamide (middle panel), or shifted to 37°C for 10 minutes before diamide addition (right panel). Panels (a, b) show regions where PolII is recruited to diamide-specific genes despite being at the rpb1-1 restrictive temperature, whereas (c) shows diamide-specific genes that fail to recruit PolII.