Figure 3

Widespread occurrence of antisense transcription in C. albicans. (a, b) Scatter plots demonstrating the dependence of AS signal intensity on the sense expression level. Signal intensity of annotated feature (hyphae experiments) probes exhibiting an AS transcript expressed above the background were considered. The signals of probes representing either sense or AS transcripts for each hybridization performed without (a) or with (b) actinomycin D are plotted. (c) GO analysis of genes with recessive AS transcripts. The P-value was calculated using hypergeometric distribution, as described on the GO Term Finder website [27]. (d) Validation of dominant AS transcripts using strand-specific RT-PCR. RT-PCR analyses were performed on RNA from yeast cells using primers specific to the AS strand (+); samples were tested for endogenous RT priming and genomic DNA contamination (RT-PCR with no RT primers (-)).